Download PDF by David K. Gardner, Michelle Lane, Andrew J. Watson: A Laboratory Guide to the Mammalian Embryo

By David K. Gardner, Michelle Lane, Andrew J. Watson

ISBN-10: 0195142268

ISBN-13: 9780195142266

This publication pulls jointly the entire variety of phone tradition, biochemical, microscopic, and genetic innovations to check the early mammalian embryo. in the past, there hasn't ever been any such accomplished compendium, notwithstanding there were extra targeted books of protocol, comparable to Manipulating the Mouse Embryo, from chilly Spring Harbor. This publication is meant to entice all constituencies, from simple experimental technology to medical and animal technology purposes.

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In vitro fertilization of bovine oocytes and preparation for culture Preparation 1. Media required for in vitro fertilization of bovine oocytes: 10 ml of Sperm Talp (modified Tyrodes solution), 10 ml of modified Fertilization (fert) Talp, and 30 ml of fert HEPES medium. ) 2. Dishes for fertilization. Fertilization can occur either in large volumes of 500 ml covered with oil or in 50-ml microdrops under oil. It is preferable to always cover the dishes with oil to minimize changes in pH and osmolality that may occur.

M. (1992). J. Reprod. , 95, 349. Rose, U. , Hanssen, R. , and Kloosterboer, H. J. (1999). Biol. , 61, 503. , and Van Steirteghem, A. C. (1996). Hum. , 11, 2656. Eppig, J. , and Hirao, Y. (1997). Biol. , 56, 976. Boland, N. , Humpherson, D. , Leese, H. , and Gosden, R. G. (1993). Biol. , 48, 798. Murray, A. , Gosden, R. , and Spears, N. (1998). J. Reprod. , 113, 27. Ryle, M. (1969). J. Reprod. , 20, 307. Greenwald, G. , and Moor, R. M. (1989). J. Reprod. , 87, 561. , and Winston, R. M. (1999). Hum.

The Parthenon Publishing Group, New York. 18. , Gardner, D. , and Trounson, A. (1994). J. Reprod. Fertil. Abstr. , 13, 70. 19. Eppig, J. , and Wigglesworth, K. (1996). Mol. Reprod. , 44, 260. 20. Champlin, A. , Dorr, D. , and Gates, A. H. (1973). Biol. , 8, 491. 3 DAVID K. GARDNER MICHELLE LANE Culture of the Mammalian Preimplantation Embryo 1. EMBRYO CULTURE MEDIA There are several treatises on the composition of embryo culture media and the role of specific medium components in supporting mammalian embryo development in vitro (1–3).

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A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson

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