JOHN M. GRAHAM's Biomembrane Protocols: I. Isolation and Analysis PDF

By JOHN M. GRAHAM

ISBN-10: 0896032361

ISBN-13: 9780896032361

An excellent ebook with lots of protocols for these operating with membranes. very good for subcellular fractionation of tissues.

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The microsoma1 (vesicular) fraction of a homogenate will also contain vesicles derived from any domain of the plasma membrane that, in vivo, is not stabilized by structures, such as tight junctions, desmosomes, or an extensive cytoskeleton. Thus, the blood sinusoidal membrane of hepatocytes and the basolateral membranes of enterocytes both tend to form vesicles, whereas hepatocyte contiguous membranes and enterocyte microvillar membranes will remain intact, as long as the homogenization is relatively mild (see Chapter 6).

M. and Winterbourne, D. J. (1988) Subcellular localization of the sulphation reaction of heparan sulphate synthesis and transport of the proteoglycan to the cell surface m rat liver. Biochem. J. 252,437-445. 9. , Wattiaux-de-Coninck, S , and Wattiaux, R. , ed ), IRL at Oxford University, Oxford, UK, pp. 119-137 10. , Wattiaux-de-Coninck, S. Ronveaux-Dupal, M. , and Dubois, F. (1978) Isolation of rat liver lysosomes by rsopycmc centrifugation m a metnzamide gradient J Cell Biol. 78,349-368. 40 Graham 11, Graham, J.

Wattiaux-de-Coninck, S , and Wattiaux, R. , ed ), IRL at Oxford University, Oxford, UK, pp. 119-137 10. , Wattiaux-de-Coninck, S. Ronveaux-Dupal, M. , and Dubois, F. (1978) Isolation of rat liver lysosomes by rsopycmc centrifugation m a metnzamide gradient J Cell Biol. 78,349-368. 40 Graham 11, Graham, J. , Ford, T , and Rickwood, D. (1979) Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge. Anal. Biochem. 187,3 18-323.

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Biomembrane Protocols: I. Isolation and Analysis by JOHN M. GRAHAM


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