Read e-book online Carbohydrate analysis: high performance liquid PDF

By Ziad El Rassi

ISBN-10: 0444899812

ISBN-13: 9780444899811

Carbohydrates and glycoconjugates play an immense function in numerous lifestyles procedures. the wide range of carbohydrate species and their inherent polydispersity and heterogeneity require separation thoughts of excessive resolving energy and excessive selectivity reminiscent of excessive functionality liquid chromatography (HPLC) and capillary electrophoresis (HPCE). within the final decade HPLC, and lately HPCE tools were built for the excessive answer and reproducible quantitation of carbohydrates. regardless of the significance of those column separation applied sciences within the region of carbohydrates, no prior booklet describes really good equipment for the separation, purification and detection of carbohydrates and glycoconjugates by means of HPLC and HPCE.

Therefore, the target of the current booklet is to supply a entire overview of carbohydrate research through HPLC and HPCE via overlaying analytical and preparative separation innovations for all periods of carbohydrates together with mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in meals and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc.

The publication may be of curiosity to a large viewers, together with analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, and so on. it's going to even be an invaluable reference paintings for either the skilled analyst and the newcomer in addition to for clients of HPLC and HPCE, graduates and postdoctoral scholars.

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Step-by-step details are given in “Methods in Carbohydrate Chemistry”, Vol. V [29]. The bacteria are suspended in a mixture of equal volumes of water and 90% phenol at 65°C for -15 minutes. After cooling to -1o”C, two fluid phases form and insoluble material remains. After centrifugation, the upper (aqueous) phase is collected, plus two aqueous re-extractions of the phenol layer, and then dialyzed to remove the phenol. After this procedure the bacterial proteins are found in the phenol layer, but the RNA and lipopolysaccharide have entered the aqueous phase.

The proteoglycans contain a protein core to which polyanionic glycosaminoglycan chains are attached. The general structures of the glycosaminoglycans and their attachment sites to proteins are given in Fig. 3. Aggregating proteoglycan, called aggrecan, the major cartilage proteoglycan, forms enormous macromolecular complexes with hyaluronic acid. Binding occurs via the hyaluronic acid-binding region of the aggrecan core protein and is stabilized by link protein. Approximately 100 chondroitin sulfate chains with molecular weights around 20,000 are linked to the core protein of aggrecan via xylosyl-serine linkages, and a smaller number of keratan sulfate chains are linked via N-acetylgalactosamine-serine or -threonine linkages.

References pp. 35-37 16 Chapter I The homogenized cell walls are then washed with buffer to remove intracellular particles and proteins and the phenol, if it was used, and then rinsed with organic solvents to remove lipids. Whatever remains insoluble is considered cell wall material. Clearly it is very difficult to prove that a particular minor component found in this material was truly a part of the cell wall before the homogenization. There are many schemes for solubilization of cell wall polymers.

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Carbohydrate analysis: high performance liquid chromatography and capillary electrophoresis by Ziad El Rassi

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