By Bernard L. Horecker, Earl R. Stadtman
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46. , Bianco, A. , and Gavin, J. R. Ill,Proc. Nat. Mtg. , Abstr. p. 343A (1976). 47. Lesniak, M. , J. Biol. Chem. (1976), 251, 3720-3729. 48. Levey, G. , Adv. Cyclic Nucleotide Res. 5, 53-66 (1975). HORMONE RECEPTORS AS REGULATORS OF HORMONE ACTION 37 49. , and Angeletti, P. ,Physiol. Rev. 48, 534-569 (1968). 50. , in "Surface Membrane Receptors: Interface Between Cells and Their Environment" (R. Bradshaw, W. Frazier, R. Merrell, D. Gottlieb, and R. ), pp. 405-418. Plenum, New York, 1976. 51. Lockwood, D.
This procedure apparently afforded two advantages: (a) since the protein was dena tured and unfolded, it allowed an increased number of attachment sites of reactive protein groups with the resin and, (b) any opportunity for noncovalent attachment of NGF to the resin which might allow sub sequent release and diffusion was completely eliminated. The second interesting feature of this study resulted from the nature of the bioassay for NGF. In this assay, intact dorsal root ganglia consisting of several thousand cells are explanted in semi-solid culture with NGF to be tested.
Acad. Sci. A. 71, 84-88 (1974). 30. Ginsberg, B. , Cohen, R. , and Kahn, C. ,Proc. Am. Diabetes Assoc, Abstr. 5, 322 (1976). 31. Goldfine, I. , and Smith, G. ,Proc. Nat. Acad. Sci. A. 73, 1427-1431 (1976). 32. Hendry, I. , and Iversen, L. , Brain Res. 29, 159-162 (1971). 33. Hendry, I. , Brain Res. 82, 117-128 (1974). 34. Hendry, I. , and Iverson, L. , Brain Res. 68, 103-121 (1974). 35. , and Shooter, E. , J. Cell Biol. 67, 118-125 (1975). 36. , Nature (London) New Biol. 232, 206-211 (1971). 37.
Current topics in cellular regulation. Vol. 12 by Bernard L. Horecker, Earl R. Stadtman